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mhc iix  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mhc iix
    Mhc Iix, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mhc+iix/anti-Myosin+heavy+chain/10__1113_slash_jp289684-118-12-18
    Average 95 stars, based on 18 article reviews
    mhc iix - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Overexpression of PRDM16 improves muscle function after rotator cuff tears
    Article Snippet: For muscle fiber type analysis, slides were fixed in 4% paraformaldehyde, washed with 0.1% phosphate-buffered saline–Triton X-100 (Sigma Aldrich Inc., St. Louis, MO, USA), and blocked in 2% bovine serum albumin for 1 hour. .. Samples were then incubated with major histocompatibility complex I (MHC-I) (DSHB BA-D5 1:600 bovine anti-mouse), MHC-IIa (DSHB SC-71 1:600 bovine anti-mouse), MHC-IIb (DSHB BF-F3 1:100 bovine anti-mouse), and MHC-IIx (DSHB 6H1 1:50 rabbit anti-mouse) at 4°C overnight. .. Slides were then rinsed and incubated with secondary antibodies for goat anti-mouse IgG2b Alexa Fluor 488 (cat. no. 115-547-187,1:200; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA), goat ant-mouse IgG1 DyLight 405 (cat. no. 115-477-185,1:200; Jackson ImmunoResearch Laboratories Inc.), goat anti-mouse IgM Alexa Fluor 594 (cat. no. 115-585-020,1:200; Jackson ImmunoResearch Laboratories Inc.) at 1 hour at room temperature.

    Article Title: Histological analysis of the gastrocnemius muscle in preschool and school age children with cerebral palsy compared with age-matched typically developing children.
    Article Snippet: Inconsistent alterations in skeletal muscle histology have been reported in adolescents with cerebral palsy (CP) and whether alterations are present in young children and differ from older children is not yet known.. This study aimed to define histological alterations in the medial gastrocnemius (MG) of ambulant CP (gross-motor classification system, GMFCS I-III) stratified in two age groups (preschool children, PS: 2–5 and school age children, SA: 6–9-yr old) compared with age-matched typically developing (TD) children.. We hypothesized that alterations in muscle microscopic properties are already present in PS-CP and are GMFCS level specific.

    Article Title: Overexpression of PRDM16 Improves Muscle Function after Rotator Cuff Tears.
    Article Snippet: .. Samples were then 122 incubated with MHC-I (DSHB BA-D5 1:600 bovine anti-mouse), MHC-IIa (DSHB SC-71 1:600 123 bovine anti-mouse), MHC-IIb (DSHB BF-F3 1:100 bovine anti-mouse), MHC-IIx (DSHB 6H1 124 1:50 rabbit anti-mouse) at 4C overnight.12 Slides were then rinsed and incubated with secondary 125 antibodies for goat anti-mouse IgG2b Alexa Fluor 488 (Jackson ImmunoResearch Laboratories 126 Inc., 115-547-187,1:200), goat anti-mouse IgG1 Dylight 405 (Jackson ImmunoResearch 127 Laboratories Inc., 115-477-185,1:200), goat anti-mouse IgM Alexa Fluor 594 (Jackson 128 ImmunoResearch Laboratories Inc., 115-585-020,1:200) at 1 hour at room temperature. ..

    Article Title: Histological analysis of the medial gastrocnemius muscle in young healthy children
    Article Snippet: The biopsies were cut in 5 μm thick cryosections with a cryostat at −20°C (CryostarTM NX 70 Thermo Fisher Scientific), and then transferred on a charged slide (Superfrost plus, VWR) and stored at −20°C. .. The slides were incubated for 1 h at room temperature with a 10% goat serum blocking solution (Thermo Fisher Scientific) followed by an overnight incubation at 4°C with a primary antibody cocktail against laminin (Abcam, ab11575), myosin heavy chain (MHC)-I (BA-F8,DSHB), MHC-IIa (SC-71, DSHB), MHC-IIx (6H1, DSHB) (adapted from ). .. Hereafter, the slides were washed 3 × 5 min with 1xPBS (7011044, Thermo Fisher Scientific) and incubated with a secondary antibody cocktail (Alexa Fluor 680, 350, 488, and 555, Thermo Fisher Scientific) for 1 h at room temperature.

    Article Title: Microscopic changes in the multifidus muscle in people with low back pain associated with lumbar disc herniation
    Article Snippet: .. The sections were then incubated with primary antibodies specific to MHC I (BA.D5 IgG2b), MHC IIA (SC.71 IgG1) and MHC IIX (6H1 IgM) (Developmental Studies Hybridoma Bank, Iowa City, IA, USA) without dilution for 90 min followed by three 5- minute washes in 1xPBS. ..

    Immunopeptidomics:

    Article Title: Overexpression of PRDM16 improves muscle function after rotator cuff tears
    Article Snippet: For muscle fiber type analysis, slides were fixed in 4% paraformaldehyde, washed with 0.1% phosphate-buffered saline–Triton X-100 (Sigma Aldrich Inc., St. Louis, MO, USA), and blocked in 2% bovine serum albumin for 1 hour. .. Samples were then incubated with major histocompatibility complex I (MHC-I) (DSHB BA-D5 1:600 bovine anti-mouse), MHC-IIa (DSHB SC-71 1:600 bovine anti-mouse), MHC-IIb (DSHB BF-F3 1:100 bovine anti-mouse), and MHC-IIx (DSHB 6H1 1:50 rabbit anti-mouse) at 4°C overnight. .. Slides were then rinsed and incubated with secondary antibodies for goat anti-mouse IgG2b Alexa Fluor 488 (cat. no. 115-547-187,1:200; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA), goat ant-mouse IgG1 DyLight 405 (cat. no. 115-477-185,1:200; Jackson ImmunoResearch Laboratories Inc.), goat anti-mouse IgM Alexa Fluor 594 (cat. no. 115-585-020,1:200; Jackson ImmunoResearch Laboratories Inc.) at 1 hour at room temperature.

    Article Title: Differential pathology and susceptibility to MBNL loss across muscles in myotonic dystrophy mouse models
    Article Snippet: .. Antibodies included the following: Laminin (MilliporeSigma, L9393, 1:1,000), eMHC/ MYH3 (Developmental Studies Hybridoma Bank [DSHB], F1.652-s, 1:40), MHC-I (DSHB, BA-F8-c, 1:400), MHC-IIA (DSHB, SC-71-c, 1:300), MHC-IIB (DSHB, BF-F3-c, 1:200), MHC-IIX (DSHB, 6H1-s, 1:20), DAPI (MilliporeSigma, D9542, 1:25,000), and Perilipin (Cell Signaling Technology, 9349S, 1:1000). ..

    Article Title: Histological analysis of the gastrocnemius muscle in preschool and school age children with cerebral palsy compared with age-matched typically developing children.
    Article Snippet: Inconsistent alterations in skeletal muscle histology have been reported in adolescents with cerebral palsy (CP) and whether alterations are present in young children and differ from older children is not yet known.. This study aimed to define histological alterations in the medial gastrocnemius (MG) of ambulant CP (gross-motor classification system, GMFCS I-III) stratified in two age groups (preschool children, PS: 2–5 and school age children, SA: 6–9-yr old) compared with age-matched typically developing (TD) children.. We hypothesized that alterations in muscle microscopic properties are already present in PS-CP and are GMFCS level specific.

    Article Title: Overexpression of PRDM16 Improves Muscle Function after Rotator Cuff Tears.
    Article Snippet: .. Samples were then 122 incubated with MHC-I (DSHB BA-D5 1:600 bovine anti-mouse), MHC-IIa (DSHB SC-71 1:600 123 bovine anti-mouse), MHC-IIb (DSHB BF-F3 1:100 bovine anti-mouse), MHC-IIx (DSHB 6H1 124 1:50 rabbit anti-mouse) at 4C overnight.12 Slides were then rinsed and incubated with secondary 125 antibodies for goat anti-mouse IgG2b Alexa Fluor 488 (Jackson ImmunoResearch Laboratories 126 Inc., 115-547-187,1:200), goat anti-mouse IgG1 Dylight 405 (Jackson ImmunoResearch 127 Laboratories Inc., 115-477-185,1:200), goat anti-mouse IgM Alexa Fluor 594 (Jackson 128 ImmunoResearch Laboratories Inc., 115-585-020,1:200) at 1 hour at room temperature. ..

    Article Title: Histological analysis of the medial gastrocnemius muscle in young healthy children
    Article Snippet: The biopsies were cut in 5 μm thick cryosections with a cryostat at −20°C (CryostarTM NX 70 Thermo Fisher Scientific), and then transferred on a charged slide (Superfrost plus, VWR) and stored at −20°C. .. The slides were incubated for 1 h at room temperature with a 10% goat serum blocking solution (Thermo Fisher Scientific) followed by an overnight incubation at 4°C with a primary antibody cocktail against laminin (Abcam, ab11575), myosin heavy chain (MHC)-I (BA-F8,DSHB), MHC-IIa (SC-71, DSHB), MHC-IIx (6H1, DSHB) (adapted from ). .. Hereafter, the slides were washed 3 × 5 min with 1xPBS (7011044, Thermo Fisher Scientific) and incubated with a secondary antibody cocktail (Alexa Fluor 680, 350, 488, and 555, Thermo Fisher Scientific) for 1 h at room temperature.

    Article Title: Microscopic changes in the multifidus muscle in people with low back pain associated with lumbar disc herniation
    Article Snippet: .. The sections were then incubated with primary antibodies specific to MHC I (BA.D5 IgG2b), MHC IIA (SC.71 IgG1) and MHC IIX (6H1 IgM) (Developmental Studies Hybridoma Bank, Iowa City, IA, USA) without dilution for 90 min followed by three 5- minute washes in 1xPBS. ..

    Cell-Signaling:

    Article Title: Differential pathology and susceptibility to MBNL loss across muscles in myotonic dystrophy mouse models
    Article Snippet: .. Antibodies included the following: Laminin (MilliporeSigma, L9393, 1:1,000), eMHC/ MYH3 (Developmental Studies Hybridoma Bank [DSHB], F1.652-s, 1:40), MHC-I (DSHB, BA-F8-c, 1:400), MHC-IIA (DSHB, SC-71-c, 1:300), MHC-IIB (DSHB, BF-F3-c, 1:200), MHC-IIX (DSHB, 6H1-s, 1:20), DAPI (MilliporeSigma, D9542, 1:25,000), and Perilipin (Cell Signaling Technology, 9349S, 1:1000). ..

    other:

    Article Title: Short-Term Effects of Botulinum Toxin-A Injection on the Medial Gastrocnemius Histological Features in Ambulant Children with Cerebral Palsy: A Longitudinal Pilot Study
    Article Snippet: mhc-IIx , 1:10 , 6H1, DSHB, Iowa, USA , Alexa Fluor 555 , 1:500 , #A-21426, Invitrogen Thermo Fisher Scientific, Dilbeek, Belgium.

    Article Title: Resistance training load does not determine resistance training‐induced hypertrophy across upper and lower limbs in healthy young males
    Article Snippet: figure legend In healthy young males, we found that skeletal muscle hypertrophy following a period of resistance exercise training (RET) was consistent within and between subjects, as assessed using a variety of established methods.. The skeletal muscle hypertrophic response was independent of external load.. Changes in myofibrillar muscle protein synthesis (MyoPS) rates were comparable between higher and lower loads but were blunted following a period of RET despite progressive overload.

    Blocking Assay:

    Article Title: Histological analysis of the gastrocnemius muscle in preschool and school age children with cerebral palsy compared with age-matched typically developing children.
    Article Snippet: Inconsistent alterations in skeletal muscle histology have been reported in adolescents with cerebral palsy (CP) and whether alterations are present in young children and differ from older children is not yet known.. This study aimed to define histological alterations in the medial gastrocnemius (MG) of ambulant CP (gross-motor classification system, GMFCS I-III) stratified in two age groups (preschool children, PS: 2–5 and school age children, SA: 6–9-yr old) compared with age-matched typically developing (TD) children.. We hypothesized that alterations in muscle microscopic properties are already present in PS-CP and are GMFCS level specific.

    Article Title: Histological analysis of the medial gastrocnemius muscle in young healthy children
    Article Snippet: The biopsies were cut in 5 μm thick cryosections with a cryostat at −20°C (CryostarTM NX 70 Thermo Fisher Scientific), and then transferred on a charged slide (Superfrost plus, VWR) and stored at −20°C. .. The slides were incubated for 1 h at room temperature with a 10% goat serum blocking solution (Thermo Fisher Scientific) followed by an overnight incubation at 4°C with a primary antibody cocktail against laminin (Abcam, ab11575), myosin heavy chain (MHC)-I (BA-F8,DSHB), MHC-IIa (SC-71, DSHB), MHC-IIx (6H1, DSHB) (adapted from ). .. Hereafter, the slides were washed 3 × 5 min with 1xPBS (7011044, Thermo Fisher Scientific) and incubated with a secondary antibody cocktail (Alexa Fluor 680, 350, 488, and 555, Thermo Fisher Scientific) for 1 h at room temperature.



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    Image Search Results


    Journal: Cell Reports Medicine

    Article Title: Impact of physical activity on physical function, mitochondrial energetics, ROS production, and Ca 2+ handling across the adult lifespan in men

    doi: 10.1016/j.xcrm.2025.101968

    Figure Lengend Snippet:

    Article Snippet: mouse IgGM monoclonal anti-MHC type IIx , DSHB , Cat# BF-F3; RRID: AB_1157897.

    Techniques: Muscles, Recombinant, Saline, Software, Sterility, Microscopy, Adhesive, Stripping Membranes